In order to zero you will follow the instructions on the spec. In this case you will press “zero base” button and then another button “run” and wait for the results to appear on the screen.
Now you are ready to record your standards and your unknown samples. For this you will be putting your samples the same way you put the blank into the spec, and then press “run”, and wait for the results appearing on the screen. You are going to do this to all your standards one by one, the same way, pressing the button “run” and waiting for the results on the screen.
Now you have got all your results from your standards on the screen and you can use them to draw your standard curve. You will measure your unknown samples the same way and extrapolate the concentrations of the unknowns from the standard curve.